Effect on growth, sugar consumption, and aerobic ethanol fermentation of homologous expression of the sugar transporter gene Pshxt1 in the white rot fungus Phanerochaete sordida YK-624.
Identifieur interne : 000075 ( Main/Exploration ); précédent : 000074; suivant : 000076Effect on growth, sugar consumption, and aerobic ethanol fermentation of homologous expression of the sugar transporter gene Pshxt1 in the white rot fungus Phanerochaete sordida YK-624.
Auteurs : Toshio Mori [Japon] ; Ojiro Kondo [Japon] ; Akane Masuda [Japon] ; Hirokazu Kawagishi [Japon] ; Hirofumi Hirai [Japon]Source :
- Journal of bioscience and bioengineering [ 1347-4421 ] ; 2019.
Descripteurs français
- KwdFr :
- MESH :
- génétique : Phanerochaete, Transporteurs de monosaccharides.
- métabolisme : Glucose, Peroxidases, Phanerochaete, Transporteurs de monosaccharides, Xylose, Éthanol.
- Aérobiose, Fermentation.
English descriptors
- KwdEn :
- MESH :
- chemical , genetics : Monosaccharide Transport Proteins.
- chemical , metabolism : Ethanol, Glucose, Monosaccharide Transport Proteins, Peroxidases, Xylose.
- genetics : Phanerochaete.
- metabolism : Phanerochaete.
- Aerobiosis, Fermentation.
Abstract
Major facilitator superfamily (MFS) transporters are found in all organisms. Although numerous studies have examined the functions of yeast and mold MFS transporters in terms of sugar affinity and metabolic regulation, no functional analyses of MFS sugar transporters in white rot fungi have been reported. This study identified an MFS sugar transporter gene (Pshxt1) of the white rot fungus Phanerochaete sordida YK-624 expressed in liquid culture containing low concentrations of nitrogen source. Homologous expression of Pshxt1 dramatically increased the rates of glucose, fructose, mannose, and xylose consumption. Galactose consumption increased slightly but significantly. These data suggest that Pshxt1 has broad affinity for monosaccharides. In contrast, a transformant homologously expressing Pshxt1 consumed glucose in preference to xylose in wood enzymatic-digestion liquor and liquid culture. Additionally, homologous expression of Pshxt1 improved mycelial growth, aerobic ethanol production, and simultaneous aerobic saccharification and fermentation efficiency, whereas secretion of the ligninolytic enzyme manganese peroxidase was clearly decreased in the presence of glucose by Pshxt1 expression. These results suggest that Pshxt1 is involved in the repression of ligninolytic enzyme activity via carbon catabolite repression at sufficiently high glucose concentrations for activation of primary metabolism.
DOI: 10.1016/j.jbiosc.2019.04.016
PubMed: 31109876
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Electronic address: hirai.hirofumi@shizuoka.ac.jp.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529</wicri:regionArea><wicri:noRegion>Shizuoka 422-8529</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2019">2019</date><idno type="RBID">pubmed:31109876</idno><idno type="pmid">31109876</idno><idno type="doi">10.1016/j.jbiosc.2019.04.016</idno><idno type="wicri:Area/Main/Corpus">000069</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000069</idno><idno type="wicri:Area/Main/Curation">000069</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000069</idno><idno type="wicri:Area/Main/Exploration">000069</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Effect on growth, sugar consumption, and aerobic ethanol fermentation of homologous expression of the sugar transporter gene Pshxt1 in the white rot fungus Phanerochaete sordida YK-624.</title><author><name sortKey="Mori, Toshio" sort="Mori, Toshio" uniqKey="Mori T" first="Toshio" last="Mori">Toshio Mori</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529</wicri:regionArea><wicri:noRegion>Shizuoka 422-8529</wicri:noRegion></affiliation></author><author><name sortKey="Kondo, Ojiro" sort="Kondo, Ojiro" uniqKey="Kondo O" first="Ojiro" last="Kondo">Ojiro Kondo</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529</wicri:regionArea><wicri:noRegion>Shizuoka 422-8529</wicri:noRegion></affiliation></author><author><name sortKey="Masuda, Akane" sort="Masuda, Akane" uniqKey="Masuda A" first="Akane" last="Masuda">Akane Masuda</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529</wicri:regionArea><wicri:noRegion>Shizuoka 422-8529</wicri:noRegion></affiliation></author><author><name sortKey="Kawagishi, Hirokazu" sort="Kawagishi, Hirokazu" uniqKey="Kawagishi H" first="Hirokazu" last="Kawagishi">Hirokazu Kawagishi</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529</wicri:regionArea><wicri:noRegion>Shizuoka 422-8529</wicri:noRegion></affiliation></author><author><name sortKey="Hirai, Hirofumi" sort="Hirai, Hirofumi" uniqKey="Hirai H" first="Hirofumi" last="Hirai">Hirofumi Hirai</name><affiliation wicri:level="1"><nlm:affiliation>Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: hirai.hirofumi@shizuoka.ac.jp.</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529</wicri:regionArea><wicri:noRegion>Shizuoka 422-8529</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of bioscience and bioengineering</title><idno type="eISSN">1347-4421</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aerobiosis (MeSH)</term><term>Ethanol (metabolism)</term><term>Fermentation (MeSH)</term><term>Glucose (metabolism)</term><term>Monosaccharide Transport Proteins (genetics)</term><term>Monosaccharide Transport Proteins (metabolism)</term><term>Peroxidases (metabolism)</term><term>Phanerochaete (genetics)</term><term>Phanerochaete (metabolism)</term><term>Xylose (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Aérobiose (MeSH)</term><term>Fermentation (MeSH)</term><term>Glucose (métabolisme)</term><term>Peroxidases (métabolisme)</term><term>Phanerochaete (génétique)</term><term>Phanerochaete (métabolisme)</term><term>Transporteurs de monosaccharides (génétique)</term><term>Transporteurs de monosaccharides (métabolisme)</term><term>Xylose (métabolisme)</term><term>Éthanol (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Monosaccharide Transport Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Ethanol</term><term>Glucose</term><term>Monosaccharide Transport Proteins</term><term>Peroxidases</term><term>Xylose</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Phanerochaete</term><term>Transporteurs de monosaccharides</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phanerochaete</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glucose</term><term>Peroxidases</term><term>Phanerochaete</term><term>Transporteurs de monosaccharides</term><term>Xylose</term><term>Éthanol</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Aerobiosis</term><term>Fermentation</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Aérobiose</term><term>Fermentation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Major facilitator superfamily (MFS) transporters are found in all organisms. Although numerous studies have examined the functions of yeast and mold MFS transporters in terms of sugar affinity and metabolic regulation, no functional analyses of MFS sugar transporters in white rot fungi have been reported. This study identified an MFS sugar transporter gene (Pshxt1) of the white rot fungus Phanerochaete sordida YK-624 expressed in liquid culture containing low concentrations of nitrogen source. Homologous expression of Pshxt1 dramatically increased the rates of glucose, fructose, mannose, and xylose consumption. Galactose consumption increased slightly but significantly. These data suggest that Pshxt1 has broad affinity for monosaccharides. In contrast, a transformant homologously expressing Pshxt1 consumed glucose in preference to xylose in wood enzymatic-digestion liquor and liquid culture. Additionally, homologous expression of Pshxt1 improved mycelial growth, aerobic ethanol production, and simultaneous aerobic saccharification and fermentation efficiency, whereas secretion of the ligninolytic enzyme manganese peroxidase was clearly decreased in the presence of glucose by Pshxt1 expression. These results suggest that Pshxt1 is involved in the repression of ligninolytic enzyme activity via carbon catabolite repression at sufficiently high glucose concentrations for activation of primary metabolism.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" IndexingMethod="Curated" Owner="NLM"><PMID Version="1">31109876</PMID><DateCompleted><Year>2019</Year><Month>12</Month><Day>09</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>17</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1347-4421</ISSN><JournalIssue CitedMedium="Internet"><Volume>128</Volume><Issue>5</Issue><PubDate><Year>2019</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Journal of bioscience and bioengineering</Title><ISOAbbreviation>J Biosci Bioeng</ISOAbbreviation></Journal><ArticleTitle>Effect on growth, sugar consumption, and aerobic ethanol fermentation of homologous expression of the sugar transporter gene Pshxt1 in the white rot fungus Phanerochaete sordida YK-624.</ArticleTitle><Pagination><MedlinePgn>537-543</MedlinePgn></Pagination><ELocationID EIdType="pii" ValidYN="Y">S1389-1723(19)30061-1</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.jbiosc.2019.04.016</ELocationID><Abstract><AbstractText>Major facilitator superfamily (MFS) transporters are found in all organisms. Although numerous studies have examined the functions of yeast and mold MFS transporters in terms of sugar affinity and metabolic regulation, no functional analyses of MFS sugar transporters in white rot fungi have been reported. This study identified an MFS sugar transporter gene (Pshxt1) of the white rot fungus Phanerochaete sordida YK-624 expressed in liquid culture containing low concentrations of nitrogen source. Homologous expression of Pshxt1 dramatically increased the rates of glucose, fructose, mannose, and xylose consumption. Galactose consumption increased slightly but significantly. These data suggest that Pshxt1 has broad affinity for monosaccharides. In contrast, a transformant homologously expressing Pshxt1 consumed glucose in preference to xylose in wood enzymatic-digestion liquor and liquid culture. Additionally, homologous expression of Pshxt1 improved mycelial growth, aerobic ethanol production, and simultaneous aerobic saccharification and fermentation efficiency, whereas secretion of the ligninolytic enzyme manganese peroxidase was clearly decreased in the presence of glucose by Pshxt1 expression. These results suggest that Pshxt1 is involved in the repression of ligninolytic enzyme activity via carbon catabolite repression at sufficiently high glucose concentrations for activation of primary metabolism.</AbstractText><CopyrightInformation>Copyright © 2019 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mori</LastName><ForeName>Toshio</ForeName><Initials>T</Initials><AffiliationInfo><Affiliation>Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kondo</LastName><ForeName>Ojiro</ForeName><Initials>O</Initials><AffiliationInfo><Affiliation>Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Masuda</LastName><ForeName>Akane</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kawagishi</LastName><ForeName>Hirokazu</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Graduate School of Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hirai</LastName><ForeName>Hirofumi</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Faculty of Agriculture, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan; Research Institute of Green Science and Technology, Shizuoka University, 836 Ohya, Suruga-ku, Shizuoka 422-8529, Japan. Electronic address: hirai.hirofumi@shizuoka.ac.jp.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2019</Year><Month>05</Month><Day>17</Day></ArticleDate></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>J Biosci Bioeng</MedlineTA><NlmUniqueID>100888800</NlmUniqueID><ISSNLinking>1347-4421</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009004">Monosaccharide Transport Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>3K9958V90M</RegistryNumber><NameOfSubstance UI="D000431">Ethanol</NameOfSubstance></Chemical><Chemical><RegistryNumber>A1TA934AKO</RegistryNumber><NameOfSubstance UI="D014994">Xylose</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.-</RegistryNumber><NameOfSubstance UI="D010544">Peroxidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.13</RegistryNumber><NameOfSubstance UI="C051129">manganese peroxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>IY9XDZ35W2</RegistryNumber><NameOfSubstance UI="D005947">Glucose</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000332" MajorTopicYN="N">Aerobiosis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000431" MajorTopicYN="N">Ethanol</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005285" MajorTopicYN="Y">Fermentation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005947" MajorTopicYN="N">Glucose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009004" MajorTopicYN="N">Monosaccharide Transport Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010544" MajorTopicYN="N">Peroxidases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020075" MajorTopicYN="N">Phanerochaete</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014994" MajorTopicYN="N">Xylose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Aerobic ethanol fermentation</Keyword><Keyword MajorTopicYN="N">Homologous expression</Keyword><Keyword MajorTopicYN="N">Phanerocahete sordida</Keyword><Keyword MajorTopicYN="N">Sugar transporter</Keyword><Keyword MajorTopicYN="N">White-rot fungi</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>01</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2019</Year><Month>04</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2019</Year><Month>04</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2019</Year><Month>5</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>12</Month><Day>18</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>5</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">31109876</ArticleId><ArticleId IdType="pii">S1389-1723(19)30061-1</ArticleId><ArticleId IdType="doi">10.1016/j.jbiosc.2019.04.016</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country></list><tree><country name="Japon"><noRegion><name sortKey="Mori, Toshio" sort="Mori, Toshio" uniqKey="Mori T" first="Toshio" last="Mori">Toshio Mori</name></noRegion><name sortKey="Hirai, Hirofumi" sort="Hirai, Hirofumi" uniqKey="Hirai H" first="Hirofumi" last="Hirai">Hirofumi Hirai</name><name sortKey="Kawagishi, Hirokazu" sort="Kawagishi, Hirokazu" uniqKey="Kawagishi H" first="Hirokazu" last="Kawagishi">Hirokazu Kawagishi</name><name sortKey="Kondo, Ojiro" sort="Kondo, Ojiro" uniqKey="Kondo O" first="Ojiro" last="Kondo">Ojiro Kondo</name><name sortKey="Masuda, Akane" sort="Masuda, Akane" uniqKey="Masuda A" first="Akane" last="Masuda">Akane Masuda</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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